Soybean cotyledonary nodes were transformed with s gene of ibv via a . . tumefaciens - med ated transformation . the highest transformation rate of 6 . 7 % was detected in the present experiment
2.
The first two reports of soybean transformation used two very different methods to transform soybean : hinchee et al ( 1988 ) used agrobacterium - mediated transformation of cotyledonary nodes while mccabe et al ( 1988 ) used particle bombardment of shoot meristems . soybean transformation reports following these initial papers have been limited and the transformation efficiency of soybean has remained low , the reason is that cells that are regeneration - competent must also be accessible and transformation - competent
3.
( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 )
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